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HRID441477

反应详情

EQUATION

反应方程式

HRID 441477 的结构方程式

REACTANTS

反应物

4
反应SMILES：II
HCID807

Iodine

I2

反应SMILES：NC(=O)C1=CN([C@@H]2O[C@H](COP(=O)(O)OP(=O)(O)OC[C@H]3O[C@@H](n4cnc5c(N)ncnc54)[C@H](O)[C@@H]3O)[C@@H](O)[C@H]2O)C=CC1
HCID439153

Adenosine 5'-(trihydrogen diphosphate), P'→5'-ester with 1,4-dihydro-1-.beta.-D-ribofuranosyl-3-pyridinecarboxamide

C21H29N7O14P2

反应SMILES：O=C([O-])O.[Na+]
HCID516892

Sodium Bicarbonate

CHNaO3

反应SMILES：O=CC(O)C(O)C(O)C(O)COP(=O)(O)O.[Ba]
HCID16219436

Barium--6-O-phosphonohexose (1/1)

C6H13BaO9P

PRODUCTS

产物

1
反应SMILES：O=P(O)(O)OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O
HCID439427

beta-D-Glucopyranose 6-phosphate

C6H13O9P

AUXILIARIES

试剂、催化剂与溶剂

1
反应SMILES：CN(C)C=O
溶剂HCID6228

Dimethylformamide

C3H7NO

3 次

PROCEDURE

实验过程

Following the procedure described above, 10mg of the T4 methyl ester diglycolate in 0.52ml dry DMF was reacted with 1.3mg NHS and 2.3mg ECDI. To 8.7mg G-6-PDH (0.08μ mole) in 1.65ml 1mg/ml sodium bicarbonate was added 5mg G-6-P and 12.5mg NADH, followed by 0.335ml DMF, followed by 9.5μmole ester prepared above in 0.215ml DMF. In 4 hours, a 47% loss in activity was observed. The hapten number was found to be 12 by UV and 13.2 by iodine analysis.

WORKUP

后处理

  1. customwas reacted with 1.3mg NHS and 2.3mg ECDI