Iodine → Beta-D-Glucose 6-phosphate

HRID 441477Notes

Reaction equation

HRID 441477 reaction equation
C(=O)(O)[O-].[Na+].[CH2:6]([O:17][P:18]([OH:21])([OH:20])=[O:19])[CH:7]([OH:16])[CH:8]([OH:15])[CH:9]([OH:14])[CH:10]([OH:13])[CH:11]=[O:12].[Ba].C1N=C(N)C2N=CN([C@@H]3O[C@H](COP(OP(OC[C@H]4O[C@@H](N5C=C(C(N)=O)CC=C5)[C@H](O)[C@@H]4O)(O)=O)(O)=O)[C@@H](O)[C@H]3O)C=2N=1.II>CN(C=O)C>[CH2:6]([O:17][P:18]([OH:21])([OH:20])=[O:19])[C@H:7]1[O:16][C@@H:11]([OH:12])[C@H:10]([OH:13])[C@@H:9]([OH:14])[C@@H:8]1[OH:15]

Participants

6

Reactant 4

HCID 807IodineHCID 439153NadhHCID 516892Sodium BicarbonateHCID 16219436Barium--6-O-phosphonohexose (1/1)

Product 1

HCID 439427Beta-D-Glucose 6-phosphate

Reagents, catalysts & solvents 1

HCID 6228DimethylformamideSolvent3×

Procedure

Following the procedure described above, 10mg of the T4 methyl ester diglycolate in 0.52ml dry DMF was reacted with 1.3mg NHS and 2.3mg ECDI. To 8.7mg G-6-PDH (0.08μ mole) in 1.65ml 1mg/ml sodium bicarbonate was added 5mg G-6-P and 12.5mg NADH, followed by 0.335ml DMF, followed by 9.5μmole ester prepared above in 0.215ml DMF. In 4 hours, a 47% loss in activity was observed. The hapten number was found to be 12 by UV and 13.2 by iodine analysis.

Workup

  1. customwas reacted with 1.3mg NHS and 2.3mg ECDI

Sources & evidence

ORD record
ord-c08a2a382b3b4e68af4b574d4ed770bf
Source dataset
uspto-grants-1977_08
Patent number
US04040907

Reaction notes

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