2-Hydroxyethyl 2,5,7,8-tetramethyl-2-(4,8,12-trimethyltridecyl)-3,4-dihydro-2H-1-benzopyran-6-yl butanedioate → Cyclosporine TPGS

Participants

Reaction Steps

200 mg of Cyclosporine was weighed into a 100 ml beaker and 1.2 grams of Vitamin E TPGS was added, and to this was added 8 grams of ethanol. The ethanol solvent, Cyclosporine, and TPGS were stirred at room temperature for 10 minutes until the Cyclosporine and TPGS components were clearly dissolved. The solvent was evaporated from a Petri dish overnight and the sample was examined under an Olympus BX50 microscope at 200× magnification by adding a small quantity of water to the cyclosporine/TPGS, and watching for visible crystals. No crystals were observed, only a clear solution was obtained. It was therefore concluded that solvent evaporation will also work to produce clear solutions of Cyclosporine/TPGS when added to water.

Provenance & Evidence