HRID443324

反应详情

EQUATION

反应方程式

REACTANTS

反应物

HCID64774

Pyridinium fluoride

C5H6FN

结构：O=c1ccn2c(n1)O[C@H]1[C@H](O)[C@@H](CO)O[C@H]12

HCID806138

2,2′-Anhydrouridine

C9H10N2O5

PRODUCTS

生成物

结构：O=c1ccn([C@@H]2O[C@H](CO)[C@@H](O)[C@H]2F)c(=O)[nH]1

HCID150851

1-(2-Deoxy-2-fluoropentofuranosyl)-4-hydroxypyrimidin-2(1H)-one

C9H11FN2O5

收率 75%

AUXILIARIES

试剂、催化剂与溶剂

溶剂HCID31275

1,4-Dioxane

C4H8O2

PROCEDURE

实验过程

2,2'-Cyclouridine is treated with a solution of 70% hydrogen fluoride/pyridine in dioxane at 120° C. for ten hours to provide after solvent removal a 75% yield of 2'-deoxy-2'-fluorouridine. The 5'-DMT and 3'-cyanoethoxydiisopropyl-phosphoramidite derivitized nucleoside is obtained by standard literature procedures [Gait, Ed., Oligonucleotide Synthesis. A Practical Approach, IRL Press, Washington, D.C. (1984)], or according to the procedure of Example 4-a.

WORKUP

后处理

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