Hydrochloric Acid → HCID 49821990, compound

TEA (0.315 ml, 2.258 mmol) was added to a suspension of 6-methylnicotinic acid (217 mg, 1.581 mmol) and diphenylphosphoryl azide (0.343 ml, 1.581 mmol) in toluene (35 mL). The mixture was stirred at room temperature for 1 h, then heated to reflux for 1 h, and then cooled to room temperature. A solution of 2-(6-chloropyridin-3-yl)-4-{[(2S)-1-methoxypropan-2-yl]oxy}-5,6,7,8-tetrahydropyrido-[4,3-d]pyrimidine (P-4, 504 mg, 1.505 mmol) and TEA (0.315 ml, 2.258 mmol) in DCM (10 mL) was added to the mixture and the stirring was continued at room temperature for 20 min. The mixture was partitioned between EtOAc (30 mL) and sat. aq. NaHCO3 (30 mL). The organic phase was washed with brine, dried over Na2SO4, filtered, and concentrated in vacuo. The resulting residue was purified by gradient elution on silica gel 0 to 70% [10% MeOH in DCM] in DCM. The pale yellow amorphous free base was dissolved in 15 mL DCM and treated with 1 N HCl in diethyl ether (1.505 ml, 3.01 mmol). The resulting suspension was stirred at room temperature for 10 min and then concentrated in vacuo. The solids were suspended in diethyl (15 mL), stirred for 20 min, and then filtered through a scintered glass funnel. The isolated solid was dried under high vacuum to afford the title compound (528 mg, 69%) as an off-white solid. Data for P-5: 1H NMR (500 MHz, CDCl3) δ 9.35 (d, J=2.2 Hz, 1H), 8.61 (dd, J=8.3, 1.6 Hz, 1H), 8.39 (d, J=2.7 Hz, 1H), 7.89 (dd, J=8.3, 2.7 Hz, 1H), 7.41 (d, J=8.5 Hz, 1H), 7.12 (d, J=8.6 Hz, 1H), 6.81 (s, 1H), 5.69 (m, 1H), 4.58 (s, 2H), 3.87 (m, 2H), 3.69 (dd, J=10.5, 6.4 Hz, 1H), 3.60 (dd, 10.5, 3.7 Hz, 1H), 3.42 (s, 3H), 3.03 (t, J=5.6 Hz, 2H), 2.52 (s, 3H), 1.43 (d, J=6.34, Hz, 3H); HRMS m/z (M+H) 469.1753 found, 469.1749 required.

1. temperatureheated
2. temperatureto reflux for 1 h
3. temperaturecooled to room temperature
4. waitthe stirring was continued at room temperature for 20 min
5. customThe mixture was partitioned between EtOAc (30 mL) and sat. aq. NaHCO3 (30 mL)
6. washThe organic phase was washed with brine
7. dry with materialdried over Na2SO4
8. filtrationfiltered
9. concentrationconcentrated in vacuo
10. customThe resulting residue was purified by gradient elution on silica gel 0 to 70% [10% MeOH in DCM] in DCM
11. dissolutionThe pale yellow amorphous free base was dissolved in 15 mL DCM
12. stirringThe resulting suspension was stirred at room temperature for 10 min
13. concentrationconcentrated in vacuo
14. stirringstirred for 20 min
15. filtrationfiltered through a scintered glass funnel
16. customThe isolated solid was dried under high vacuum

ORD record

ord-59d766bcd7c7468a8c54530a151d4b88

Source dataset

uspto-grants-2013_07

DOI

10.6084/m9.figshare.5104873.v1

Patent number

US08492392B2