反应详情

trans-N-tert-Butoxycarbonyl-1,2-cyclopentane-diamine (1.40 g) was dissolved in N,N-dimethylformamide (15 ml), and to the solution 5-chloroindole-2-carboxylic acid (1.64 g), 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (2.68 g) and 1-hydroxybenzotriazole monohydrate (473 mg) were added. The mixture was stirred at room temperature for 23 hours. The solvent was distilled off under reduced pressure, and dichloromethane and a saturated solution of sodium hydrogencarbonate were added to the residue to collect precipitates by filtration. The precipitates were washed with ethyl acetate, dichloromethane and methanol. On the other hand, the filtrate was separated to give an organic layer,which was taken out and dried over anhydrous sodium sulfate, and the solvent was then distilled off under reduced pressure. The residue was purified by medium-pressure flash column chromatography on silica gel (dichloromethane:methanol=19:1) to obtain a pale yellow solid. This pale yellow solid was combined with the precipitates obtained by the filtration and dissolved in dichloromethane (10 ml), and trifluoroacetic acid (10 ml) was added to stir the mixture at room temperature for 3 hours. The solvent was distilled off under reduced pressure, and dichloromethane and a 1N aqueous solution of sodium hydroxide were added to the residue to collect precipitate by filtration. The organic layer of the filtrate was separated and dried over anhydrous sodium sulfate. The precipitates collected by the filtration were added to this solution, and a 4N dioxane solution (20 ml) of hydrochloric acid was further added. The solvent was distilled off under reduced pressure, and dichloromethane (10 ml) and a 4N dioxane solution (10 ml) of hydrochloric acid were added to the residue. The solvent was distilled off again under reduced pressure. Ethyl acetate was added to the residue to collect precipitates by filtration, thereby obtaining the title compound (1.83 g) as a gray solid.

1. distillationThe solvent was distilled off under reduced pressure, and dichloromethane
2. additiona saturated solution of sodium hydrogencarbonate were added to the residue
3. customto collect
4. customprecipitates by filtration
5. washThe precipitates were washed with ethyl acetate, dichloromethane and methanol
6. customOn the other hand, the filtrate was separated
7. customto give an organic layer,which
8. dry with materialdried over anhydrous sodium sulfate
9. distillationthe solvent was then distilled off under reduced pressure
10. customThe residue was purified by medium-pressure flash column chromatography on silica gel (dichloromethane:methanol=19:1)
11. customto obtain a pale yellow solid
12. customobtained by the filtration
13. stirringto stir the mixture at room temperature for 3 hours
14. distillationThe solvent was distilled off under reduced pressure, and dichloromethane
15. additiona 1N aqueous solution of sodium hydroxide were added to the residue
16. customto collect precipitate
17. filtrationby filtration
18. customThe organic layer of the filtrate was separated
19. dry with materialdried over anhydrous sodium sulfate
20. filtrationThe precipitates collected by the filtration
21. additionwere added to this solution
22. additiona 4N dioxane solution (20 ml) of hydrochloric acid was further added
23. distillationThe solvent was distilled off under reduced pressure, and dichloromethane (10 ml)
24. additiona 4N dioxane solution (10 ml) of hydrochloric acid were added to the residue
25. distillationThe solvent was distilled off again under reduced pressure
26. additionEthyl acetate was added to the residue
27. customto collect
28. customprecipitates by filtration