反应详情

温度

23 °C

NAD+ (34.0 mg, 38.0 μmol) was placed in 2 mL of a 0.1 M phosphate buffer. The pH of this solution was adjusted to 8.1 with NaOH and then placed in an 80 mL Fisher-Porter bottle. The Fisher-Porter bottle was evacuated and refilled with argon three times then [Cl2Ru(TPPTS)2]2 (6.9 mg, 2.6 μmol) was added under a flow of argon. The Fisher-Porter bottle was evacuated and filled with 70 psi of H2 and stirred to 23° C. for 17.0 hr. The volatiles were removed under reduced pressure and a sample dissolved in D2O To this was added 20 μl of trimethylsilylpropionate standard solution. 1H NMR integration shows that 9.2 μmole of NADH have been produced under these conditions.

1. customThe Fisher-Porter bottle was evacuated
2. additionrefilled with argon three times
3. customThe Fisher-Porter bottle was evacuated
4. additionfilled with 70 psi of H2
5. customThe volatiles were removed under reduced pressure
6. dissolutiona sample dissolved in D2O To
7. additionthis was added 20 μl of trimethylsilylpropionate standard solution