反应详情

温度

35 °C

Next, 100 ml of a nutrient medium (pH 7) containing 3% of ammonium fumarate, 0.2% of dipotassium phosphate, 0.025% of magnesium sulfate, 4% of corn steep liquor, and 0.05% of calcium carbonate was inoculated with Escherichia coli and then shaken at 35° C. for 24 hours. Thereafter, 50 ml of the resulting culture of Eschericia coli and 50 ml of a fresh nutrient medium were added to the aforesaid gel-containing flask, which was shaken at 35° C. for 24 hours. After removal of the supernatant, the gel was washed three times with 50-ml portions of sterilized water. Then, 100 ml of a fresh nutrient medium was added to the gel-containing flask, which was shaken at 35° C. As a result, aspartic acid was produced at a rate of 872 μmol/hr/g of gel.

1. additionThereafter, 50 ml of the resulting culture of Eschericia coli and 50 ml of a fresh nutrient medium were added to the aforesaid
2. additiongel-containing flask
3. stirringwhich was shaken at 35° C. for 24 hours
4. customAfter removal of the supernatant
5. washthe gel was washed three times with 50-ml portions of sterilized water
6. additionThen, 100 ml of a fresh nutrient medium was added to the gel-containing flask, which
7. stirringwas shaken at 35° C