Calcium Carbonate → L-Aspartic Acid
Reaction equation
[C:1]([O-:8])(=[O:7])/[CH:2]=[CH:3]/[C:4]([O-:6])=[O:5].[NH4+:9].[NH4+].P(O)([O-])([O-])=O.[K+].[K+].S([O-])([O-])(=O)=O.[Mg+2].C(=O)([O-])[O-].[Ca+2]>>[NH2:9][C@H:2]([C:1]([OH:8])=[O:7])[CH2:3][C:4]([OH:6])=[O:5]Participants
Conditions
- Temperature
- 35 °C
Procedure
Next, 100 ml of a nutrient medium (pH 7) containing 3% of ammonium fumarate, 0.2% of dipotassium phosphate, 0.025% of magnesium sulfate, 4% of corn steep liquor, and 0.05% of calcium carbonate was inoculated with Escherichia coli and then shaken at 35° C. for 24 hours. Thereafter, 50 ml of the resulting culture of Eschericia coli and 50 ml of a fresh nutrient medium were added to the aforesaid gel-containing flask, which was shaken at 35° C. for 24 hours. After removal of the supernatant, the gel was washed three times with 50-ml portions of sterilized water. Then, 100 ml of a fresh nutrient medium was added to the gel-containing flask, which was shaken at 35° C. As a result, aspartic acid was produced at a rate of 872 μmol/hr/g of gel.
Workup
- additionThereafter, 50 ml of the resulting culture of Eschericia coli and 50 ml of a fresh nutrient medium were added to the aforesaid
- additiongel-containing flask
- stirringwhich was shaken at 35° C. for 24 hours
- customAfter removal of the supernatant
- washthe gel was washed three times with 50-ml portions of sterilized water
- additionThen, 100 ml of a fresh nutrient medium was added to the gel-containing flask, which
- stirringwas shaken at 35° C
Sources & evidence
- ORD record
- ord-06aa4e7734a546fc98274d40d800ff6b
- Source dataset
- uspto-grants-1985_10
- Patent number
- US04547463
Reaction notes
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