反应详情

温度

0 °C

DIPEA (0.16 mL, 0.97 mmol) was added to a solution of Intermediate 5 (150 mg, 0.45 mmol) in DCM (6 mL). The reaction mixture was cooled to 0° C. under nitrogen atmosphere. 3-Methylisoxazole-5-carbonyl chloride (79 mg, 0.54 mmol) was then added dropwise and stirring was continued for 1 h at room temperature. The reaction was washed with sodium bicarbonate and then twice with water. The organic layer was dried over a magnesium sulphate plug and evaporated. The residue was purified by preparative HPLC (RP Shandon Hyperprep C18 BDS—8 μm, 250 g, 5 cm; mobile phase (0.25% NH4HCO3 solution in water, CH3CN)), yielding 127 mg (64%) of Compound 4.

1. washThe reaction was washed with sodium bicarbonate
2. dry with materialThe organic layer was dried over a magnesium sulphate plug
3. customevaporated
4. customThe residue was purified by preparative HPLC (RP Shandon Hyperprep C18 BDS—8 μm, 250 g, 5 cm; mobile phase (0.25% NH4HCO3 solution in water, CH3CN))