Water → HCID 88876316, compound

HRID 476219Notes

Reaction equation

HRID 476219 reaction equation
[CH2:1]([CH:3]1[CH:20]([OH:21])[CH:19]([CH3:22])[CH:18]=[C:17]([CH3:23])[CH:16]=[C:15]([O:24][CH3:25])[C:14](=[O:26])[O:13][CH:12]([CH:27]([CH:29]([OH:48])[CH:30]([CH3:47])/[C:31](=[N:41]\[O:42][CH2:43][C:44](O)=[O:45])/[CH:32]=[CH:33]/[CH:34]([CH3:40])[CH:35]([OH:39])/[CH:36]=[CH:37]/[CH3:38])[CH3:28])[CH:11]([O:49][CH3:50])[CH:10]=[CH:9][CH:8]=[C:7]([CH3:51])[CH2:6][CH:5]([CH3:52])[CH:4]1[OH:53])[CH3:2].C1C=CC2N(O)N=NC=2C=1.[NH:64]1[CH2:69][CH2:68][CH2:67][CH2:66][CH2:65]1.O>ClCCl>[OH:48][CH:29]([CH:30]([CH3:47])/[C:31](=[N:41]\[O:42][CH2:43][C:44](=[O:45])[N:64]1[CH2:69][CH2:68][CH2:67][CH2:66][CH2:65]1)/[CH:32]=[CH:33]/[CH:34]([CH3:40])[CH:35]([OH:39])/[CH:36]=[CH:37]/[CH3:38])[CH:27]([CH:12]1[O:13][C:14](=[O:26])[C:15]([O:24][CH3:25])=[CH:16][C:17]([CH3:23])=[CH:18][CH:19]([CH3:22])[CH:20]([OH:21])[CH:3]([CH2:1][CH3:2])[CH:4]([OH:53])[CH:5]([CH3:52])[CH2:6][C:7]([CH3:51])=[CH:8][CH:9]=[CH:10][CH:11]1[O:49][CH3:50])[CH3:28]

Participants

6

Reactant 4

HCID 962WaterHCID 8082PiperidineHCID 757711-HydroxybenzotriazoleHCID 88876342HCID 88876342, compound

Product 1

HCID 88876316HCID 88876316, compound

Reagents, catalysts & solvents 1

HCID 6344DichloromethaneSolvent

Procedure

To a solution of compound of example 10 (10 mg) in dichloromethane (2 mL) dicyclohexylcarbodiimide (3 mg), and HOBt (2 mg) were added. After 20 min, piperidine (1.3 mg) was added. The reaction mixture was stirred for 18 h under nitrogen atmosphere. Cold water was added to the reaction mixture, the organic layer was separated. Reaction mixture was extracted with dichloromethane (3×5 mL). The combined organic layer was washed with water (2×5 mL). The organic layer was dried over sodium sulphate, and was concentrated. The crude product was purified by preparative HPLC [Eurospere-100, C18 column (250 mm×8 mm), mobile phase: acetonitrile-water (1:1 isocratic)] to obtain the title compound.

Workup

  1. customthe organic layer was separated
  2. customReaction mixture
  3. extractionwas extracted with dichloromethane (3×5 mL)
  4. washThe combined organic layer was washed with water (2×5 mL)
  5. dry with materialThe organic layer was dried over sodium sulphate
  6. concentrationwas concentrated
  7. customThe crude product was purified by preparative HPLC [Eurospere-100, C18 column (250 mm×8 mm), mobile phase: acetonitrile-water (1:1 isocratic)]

Sources & evidence

ORD record
ord-34420754f90b4d13849eb720b15d239f
Source dataset
uspto-grants-2014_01
Patent number
US08637683B2

Reaction notes

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