反应详情

Ethyl chloroformate (66 μL) is added dropwise to an ice-cold mixture of 4-methyl-5,6-dihydro-4H-1,3a,6-triaza-as-indacene-8-carboxylic acid hydrobromide (192 mg), triethylamine (0.33 mL), and N,N-dimethylformamide (5 mL). The mixture is stirred at 0° for 1 hour then at room temperature for 30 minutes. 2-Fluoro-4-isopropoxyaniline (66 mg) is added and the mixture is stirred at room temperature for 18 hours. The mixture is diluted with ethyl acetate (50 mL) then treated with water (25 mL), and extracted with ethyl acetate (3×50 mL). The combined organic layers are washed with water (2×20 mL) and brine (1×20 mL), dried over magnesium sulfate, and concentrated in vacuo. The residue is purified by preparative thin layer chromatography on silica gel, eluting with diethyl ether, to give a brown solid. This is treated with ethanol (2 mL) and saturated aqueous potassium carbonate solution (1 mL) and the mixture is heated at 600 for 30 minutes. The mixture is cooled to room temperature and diluted with water (3 mL) and extracted with ethyl acetate (3×30 mL). The combined organic layers are washed with brine (1×10 mL), dried over magnesium sulfate, and concentrated in vacuo. The oily residue is purified by preparative thin layer chromatography on silica gel, eluting with 10% methanol in dichloromethane, to give 4-methyl-5,6-dihydro-4H-1,3a,6-triaza-as-indacene-8-carboxylic acid (2-fluoro-4-isopropoxyphenyl)amide, pale yellow solid, 10 mg: LRMS calcd 368.40, found [M+H] 369.2.

1. waitat room temperature for 30 minutes
2. stirringthe mixture is stirred at room temperature for 18 hours
3. extractionextracted with ethyl acetate (3×50 mL)
4. washThe combined organic layers are washed with water (2×20 mL) and brine (1×20 mL)
5. dry with materialdried over magnesium sulfate
6. concentrationconcentrated in vacuo
7. customThe residue is purified by preparative thin layer chromatography on silica gel
8. washeluting with diethyl ether
9. customto give a brown solid
10. temperaturethe mixture is heated at 600 for 30 minutes
11. extractionextracted with ethyl acetate (3×30 mL)
12. washThe combined organic layers are washed with brine (1×10 mL)
13. dry with materialdried over magnesium sulfate
14. concentrationconcentrated in vacuo
15. customThe oily residue is purified by preparative thin layer chromatography on silica gel
16. washeluting with 10% methanol in dichloromethane