25416-65-3 → Thyroxine

Participants

Reaction Steps

Sodium thyroxine NaT4 (36.72 mg) was dissolved in a mixture of absolute methanol (1 ml) and 0.1 N sodium hydroxide solution (0.25 ml). To the resulting solution was added I125 -T4 (radio-labeled iodinated thyroxine, 0.25 ml; total 251,610 cpm) and 0.15 M phosphate buffered saline (5.2 ml) having a pH 7.5. The resulting solution was added to a solution of human serum albumin (125 mg) in water (6.25 ml). To this was added 1-ethyl-3-(3-dimethylaminopropyl)carbondiimide (13.12 mg) and the mixture was stirred overnight at room temperature.

Provenance & Evidence