25416-65-3 → Thyroxine

HRID 482853Notes

Reaction equation

HRID 482853 reaction equation
[NH2:1][C@H:2]([C:22]([OH:24])=[O:23])[CH2:3][C:4]1[CH:11]=[C:9]([I:10])[C:8]([O:12][C:13]2[CH:20]=[C:18]([I:19])[C:17]([OH:21])=[C:15]([I:16])[CH:14]=2)=[C:6]([I:7])[CH:5]=1.[Na].OP([O-])(O)=O.OP([O-])([O-])=O.[Na+].[Na+].[Na+].[Cl-].[Cl-].[K+].[K+]>CO.[OH-].[Na+].O>[NH2:1][C@H:2]([C:22]([OH:24])=[O:23])[CH2:3][C:4]1[CH:5]=[C:6]([I:7])[C:8]([O:12][C:13]2[CH:14]=[C:15]([I:16])[C:17]([OH:21])=[C:18]([I:19])[CH:20]=2)=[C:9]([I:10])[CH:11]=1

Participants

6

Reactant 2

HCID 983225325416-65-3HCID 24978514Phosphate Buffered Saline

Product 1

HCID 5819Thyroxine

Reagents, catalysts & solvents 3

HCID 887MethanolSolventHCID 962WaterSolventHCID 14798Sodium HydroxideSolvent

Procedure

Sodium thyroxine NaT4 (36.72 mg) was dissolved in a mixture of absolute methanol (1 ml) and 0.1 N sodium hydroxide solution (0.25 ml). To the resulting solution was added I125 -T4 (radio-labeled iodinated thyroxine, 0.25 ml; total 251,610 cpm) and 0.15 M phosphate buffered saline (5.2 ml) having a pH 7.5. The resulting solution was added to a solution of human serum albumin (125 mg) in water (6.25 ml). To this was added 1-ethyl-3-(3-dimethylaminopropyl)carbondiimide (13.12 mg) and the mixture was stirred overnight at room temperature.

Workup

  1. additionTo this was added 1-ethyl-3-(3-dimethylaminopropyl)carbondiimide (13.12 mg)

Sources & evidence

ORD record
ord-cd48e19dfe744784828026b57c55d7c1
Source dataset
uspto-grants-1986_03
Patent number
US04576912

Reaction notes

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