Water → (2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal

Participants

Reaction Steps

The substrate used for the assay is a maltodextrin (Maltrin-100 available from the Grain Processing Company, Muscatine, Iowa). 0.1 ml of the enzyme solution was mixed with 0.6 ml of a 5% maltodextrin solution, 0.1 ml of 500 mM sodium acetate solution (pH 5) and water is added to make a total volume of 1 ml. After the mixture is incubated at 60° C. for 30 minutes, the reaction is terminated by immersing the mixture in a boiling water bath for 10 to 20 minutes. Glucose is then determined by the glucose-oxidase method using a glucose analyzer (Yellow Springs Instrument Company, Yellow Springs, Ohio). One glucoamylase unit is defined as the amount of enzyme required to produce 1 gram of glucose in 1 hour under the assay conditions.

Provenance & Evidence