Water → (2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal

The substrate used for the assay is a maltodextrin (Maltrin-100 available from the Grain Processing Company, Muscatine, Iowa). 0.1 ml of the enzyme solution was mixed with 0.6 ml of a 5% maltodextrin solution, 0.1 ml of 500 mM sodium acetate solution (pH 5) and water is added to make a total volume of 1 ml. After the mixture is incubated at 60° C. for 30 minutes, the reaction is terminated by immersing the mixture in a boiling water bath for 10 to 20 minutes. Glucose is then determined by the glucose-oxidase method using a glucose analyzer (Yellow Springs Instrument Company, Yellow Springs, Ohio). One glucoamylase unit is defined as the amount of enzyme required to produce 1 gram of glucose in 1 hour under the assay conditions.

1. additionis added
2. customthe reaction is terminated
3. customfor 10 to 20 minutes

ORD record

ord-bdf7470e874d4aedb3121b0007a4d7ef

Source dataset

uspto-grants-1985_08

DOI

10.6084/m9.figshare.5104873.v1

Patent number

US04536477