Thiamine Hydrochloride → L-Glutamine

An aqueous solution medium having a composition of 10% of glucose, 1% of ammonium sulfate, 0.25% of potassium primary phosphate (KH2P04), 0.04% of magnesium sulfate, 0.001% of ferrous sulfate, 350 μg/l of thiamine hydrochloride, 5 μg/l of biotin and 0.5 ml/dl of Aji-Eki® which is a soybean protein hydrolysate which accelerates the growth of L-amino acid-producing microorganisms and shortens the required culture time, at pH 7.0, was charged in an amount of 300 ml into separate small sized glass jar fermenters. After sterilizing in a conventional manner, the various L-glutamine-producing bacterial strains shown in Table 10, which had been previously grown in bouillon slants at 30° C. for 24 hours, were inoculated thereon. Then, incubation was carried out at 31.5° C. for 30 hours at 1200 rpm at an aeration rate of 1/4 volume per minute, while keeping the pH at 6.5 by the addition of ammonia gas. After completion of the fermentation, the yield of L-glutamine produced and accumulated in the solution based on glucose was determined and is shown in Table 10.

1. customproducing microorganisms
2. additionat pH 7.0, was charged in an amount of 300 ml
3. custominto separate small sized glass jar fermenters

ORD record

ord-91368b1344e648e48747bb22b5a1c04e

Source dataset

uspto-grants-1994_03

DOI

10.6084/m9.figshare.5104873.v1

Patent number

US05294547