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An aqueous solution medium having a composition of 10% of glucose, 1% of ammonium sulfate, 0.1% of potassium primary phosphate, 0.04% of magnesium sulfate, 0.001% of ferrous sulfate, 0.001% of manganese sulfate, 100 μg/l of thiamine hydrochloride, 100 μg/l of biotin and 2 ml/dl of Aji-Eki®, at pH 7.0, was charged in an amount of 300 ml into separate small sized glass jar fermenters. After sterilizing in a conventional manner, the various L-isoleucine-producing bacterial strains shown in Table 16, which had been previously grown in bouillon slants at 30° C. for 24 hours, were inoculated thereon. Then, incubation was carried out at 31.5° C. for 48 hours at 1200 rpm at an aeration rate of 1/2 volume per minute, while keeping the pH at 7.3 by the addition of ammonia gas. After completion of the fermentation, the yield of L-isoleucine produced and accumulated in the solution based on glucose was determined and is shown in Table 6.

1. additionat pH 7.0, was charged in an amount of 300 ml
2. custominto separate small sized glass jar fermenters