Acetate → Ganglioside GM1
Reaction equation
[CH3:1][CH2:2][CH2:3][CH2:4][CH2:5][CH2:6][CH2:7][CH2:8][CH2:9][CH2:10][CH2:11][CH2:12][CH2:13][CH2:14][CH2:15][CH2:16][CH2:17][C:18]([NH:20][CH2:21][CH:22]([O:40][C@@H:41]1[O:46][C@H:45]([CH2:47][OH:48])[C@@H:44]([O:49][C@H:50]2[O:55][C@@H:54]([CH2:56][OH:57])[C@@H:53]([O:58][C@H:59]3[O:64][C@@H:63]([CH2:65][OH:66])[C@@H:62]([OH:67])[C@@H:61]([O:68][C@H:69]4[O:74][C@@H:73]([CH2:75][OH:76])[C@@H:72]([OH:77])[C@@H:71]([O:78][C@]5(C(O)=O)O[C@@H](C(O)C(O[C@]6(C(O)=O)O[C@@H](C(O)C(O)CO)[C@H](N)[C@@H](O)C6)O)[C@H](N)[C@@H](O)C5)[C@@H:70]4[OH:112])[C@@H:60]3[NH:113][C:114]([CH3:116])=[O:115])[C@@H:52]([O:117][C@:118]3([C:132]([OH:134])=[O:133])[O:123][C@@H:122]([CH:124]([OH:129])[CH:125]([OH:128])[CH2:126][OH:127])[C@H:121]([NH2:130])[C@@H:120]([OH:131])[CH2:119]3)[C@@H:51]2[OH:135])[C@H:43]([OH:136])[C@H:42]1[OH:137])C(O)/C=C/CCCCCCCCCCCCC)=O.[C:138]([O-:141])(=O)[CH3:139].[OH2:142]>C(Cl)(Cl)Cl>[CH3:1][CH2:2][CH2:3][CH2:4][CH2:5][CH2:6][CH2:7][CH2:8][CH2:9][CH2:10][CH2:11][CH2:12][CH2:13][CH2:14][CH2:15][CH2:16][CH2:17][C:18]([NH:20][C@H:21]([C@H:138]([OH:141])/[CH:139]=[CH:14]/[CH2:13][CH2:12][CH2:11][CH2:10][CH2:9][CH2:8][CH2:7][CH2:6][CH2:5][CH2:4][CH2:3][CH2:2][CH3:1])[CH2:22][O:40][C@@H:41]1[O:46][C@H:45]([CH2:47][OH:48])[C@@H:44]([O:49][C@@H:50]2[O:55][C@H:54]([CH2:56][OH:57])[C@H:53]([O:58][C@@H:59]3[O:64][C@H:63]([CH2:65][OH:66])[C@H:62]([OH:67])[C@H:61]([O:68][C@@H:69]4[O:74][C@H:73]([CH2:75][OH:76])[C@H:72]([OH:77])[C@H:71]([OH:78])[C@H:70]4[OH:112])[C@H:60]3[NH:113][C:114]([CH3:116])=[O:115])[C@H:52]([O:117][C@@:118]3([C:132]([OH:134])=[O:133])[O:123][C@@H:122]([C@H:124]([OH:129])[C@H:125]([OH:128])[CH2:126][OH:127])[C@H:121]([NH:130][C:22]([CH3:21])=[O:40])[C@@H:120]([OH:131])[CH2:119]3)[C@H:51]2[OH:135])[C@H:43]([OH:136])[C@H:42]1[OH:137])=[O:142]Participants
5
Reactant 3
Product 1
Reagents, catalysts & solvents 1
Procedure
A 1 ml quantity of 0.4% ganglioside GTlb solution, 1 ml of 40 mM acetate buffer (pH 4.0) and 1 ml of distilled water were mixed together, and to the mixture was added 1 ml of aqueous solution of Isozyme S (5 U/ml). The mixture was allowed to react at 37° C. for 1 hour, and 1/10 part by volume of chloroform was added thereto to stop the enzyme reaction. Ganglioside GMl was extracted from the reaction solution with use of a mixture of chloroform and methanol (2:1) and then lyophilized, giving 2.4 mg of ganglioside GMl as a white powder.
Workup
- customto react at 37° C. for 1 hour
- customthe enzyme reaction
- extractionGanglioside GMl was extracted from the reaction solution with use of a mixture of chloroform and methanol (2:1)
Sources & evidence
- ORD record
- ord-a73071fee6d54e908f6ebd542ceebca8
- Source dataset
- uspto-grants-1994_03
- Patent number
- US05296360
Reaction notes
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