Acetate → Ganglioside GM1

Participants

Reaction Steps

A 1 ml quantity of 0.4% ganglioside GTlb solution, 1 ml of 40 mM acetate buffer (pH 4.0) and 1 ml of distilled water were mixed together, and to the mixture was added 1 ml of aqueous solution of Isozyme S (5 U/ml). The mixture was allowed to react at 37° C. for 1 hour, and 1/10 part by volume of chloroform was added thereto to stop the enzyme reaction. Ganglioside GMl was extracted from the reaction solution with use of a mixture of chloroform and methanol (2:1) and then lyophilized, giving 2.4 mg of ganglioside GMl as a white powder.

Provenance & Evidence