Sodium Carbonate → HCID 68470603, 화합물

A solution (0.5 M) of tert-butyl 5-bromo-6-cyclohexyl-4H-thieno[3,2-b]pyrrole-2-carboxylate in DMF was treated with NaH (2 eq., 60% dispersion in mineral oil) and the suspension was stirred at RT for 30 min then methyl bromoacetate (3 eq.) was added. The reaction mixture was heated at 50° C. for 1 h, then after cooling down, it was diluted with AcOEt and aqueous HCl (1 M) was added. The aqueous phase was separated and extracted with AcOEt. The combined organic phase was washed sequentially with aqueous HCl (1 N), aqueous NaHCO3 (saturated solution) and brine then dried. Evaporation of the solvent under reduced pressure gave a residue that was dissolved in DME. The resulting solution (0.2 M) was treated with 3-furylboronic acid (1.5 eq.) and aqueous Na2CO3 (1 M solution, 2 eq.), the mixture was degassed and PdCl2(dppf)2 (0.05 eq.) was added. The reaction was heated to 80° C. for 5 h under argon atmosphere. After cooling down, the reaction was treated with aqueous HCl (1 N) and extracted with AcOEt. The combined organic phase was washed with brine and dried. Evaporation of the solvent under reduced pressure gave a residue that was purified by flash chromatography on silica gel (1:9 AcOEt/petroleum ether) affording tert-butyl 6-cyclohexyl-5-(3-furyl)-4-(2-methoxy-2-oxoethyl)-4H-thieno[3,2-b]pyrrole-2-carboxylate that was dissolved in MeOH. The resulting solution (0.1 M) was treated with aqueous NaOH (2 N solution, 6 eq.). The reaction mixture was heated to reflux for 2 h. After cooling down, the reaction was concentrated and acidified to pH 1 with aqueous HCl (6 N). The acidic layer was extracted with AcOEt and the combined organic phase was washed with HCl (1 M) and brine, then dried. Evaporation of the solvent under reduced pressure gave a residue that was dissolved in DCM. The resulting solution (0.1 M) was treated with DIEA (2.5 eq.), 1-isopropyl-N-methylpyrrolidin-3-amine (1.1 eq.) and HATU (1.5 eq.). The reaction mixture was stirred at RT overnight, then it was diluted with AcOEt and washed sequentially with aqueous NaHCO3 (saturated solution) and brine then dried. Evaporation of the solvent under reduced pressure gave a residue that was treated with methanolic HCl (1.2 M solution, 25 eq.). The resulting solution (0.1 M) was stirred at RT for 2 h. Evaporation of the solvent under reduced pressure gave a residue that was purified by RP-HPLC (Conditions: Waters X-TERRA MS C18, 5 micron, 19×150 mm; flow: 20 mL/min; Gradient: A: H2O+0.1% TFA; B: MeCN+0.1% TFA; linear from 95% A to 5% A in 10 min) to afford the title compound (14%) as its TFA salt.

1. temperatureThe reaction mixture was heated at 50° C. for 1 h
2. temperatureafter cooling down
3. additionwas added
4. customThe aqueous phase was separated
5. extractionextracted with AcOEt
6. washThe combined organic phase was washed sequentially with aqueous HCl (1 N), aqueous NaHCO3 (saturated solution) and brine
7. customthen dried
8. customEvaporation of the solvent under reduced pressure
9. customgave a residue that
10. customthe mixture was degassed
11. temperatureThe reaction was heated to 80° C. for 5 h under argon atmosphere
12. temperatureAfter cooling down
13. extractionextracted with AcOEt
14. washThe combined organic phase was washed with brine
15. customdried
16. customEvaporation of the solvent under reduced pressure
17. customgave a residue that
18. customwas purified by flash chromatography on silica gel (1:9 AcOEt/petroleum ether)

ORD 기록

ord-59dab847e81b420daa2efe25d94bf3c3

출처 데이터셋

uspto-grants-2010_08

DOI

10.6084/m9.figshare.5104873.v1

특허 번호

US07781431B2