反应详情

The solution of 0.024 g of tert-butyl (1(S)-(1(S)-hydroxy-2-piperidin-1-ylethyl)-3(S)-{[2-(3-methoxypropoxy)benzoylamino]methyl}-4-methylpentyl)carbamate in 1 ml of 4M HCl (in dioxane) is stirred at 0° C. over 3 hours and subsequently concentrated by evaporation to dryness—the residue is dissolved in 1 ml of tert-butanol, frozen and lyophilized under high vacuum. The title compound is obtained as a white powder. Rf=0.09 (200:20:1 dichloromethane-methanol-25% conc. ammonia); Rt=2.95 (gradient I).

1. concentrationsubsequently concentrated by evaporation to dryness—the residue
2. dissolutionis dissolved in 1 ml of tert-butanol
3. temperaturefrozen