反应详情
EQUATION
反应方程式
REACTANTS
反应物
Distearoylphosphatidylethanolamine
C41H82NO8P
1,2-Bis(dimethylphosphino)ethane
C6H16P2
2 次
2-(4-Benzylphenoxy)-N,N-diethylethan-1-amine--hydrogen chloride (1/1)
C19H26ClNO
3-{[(2-Aminoethoxy)(hydroxy)phosphoryl]oxy}-2-(hexadecanoyloxy)propyl hexadecanoate
C37H74NO8P
1,2-Distearoyl-sn-glycero-3-phosphoethanolamine
C41H82NO8P
未命名化合物
Dimyristoylphosphatidylethanolamine
C33H66NO8P
PRODUCTS
生成物
AUXILIARIES
试剂、催化剂与溶剂
PROCEDURE
实验过程
PE lipids (1,2-dilauroyl-sn-glycero-3-phosphoethanolamine, DMPE; 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine, DMPE; 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, DPPE; 1,2-dioctadecanoyl-sn-glycero-3-phosphoethanolamine, DSPE, Avanti polar lipids. Inc.) were dissolved in 500 uL CHCl3 and 500 uL DMF, 1.2 eq of fluorescein-PEG2000-NHS (creative PEG works Inc.) was added and the reaction mixture were agitated overnight, the amphiphilic fluorescein PEG amphiphiles were purified by reverse phase HPLC using a C4 column (BioBasic-4, 200 mm×4.6 mm, Thermo Scientific), 100 mM triethylamine-acetic acid buffer (TEAA, pH 7.5)-methanol (0-30 min, 10-100%) as an eluent.
WORKUP
后处理
- customthe amphiphilic fluorescein PEG amphiphiles were purified by reverse phase HPLC
- customa C4 column (BioBasic-4, 200 mm×4.6 mm, Thermo Scientific), 100 mM triethylamine-acetic acid buffer (TEAA, pH 7.5)-methanol (0-30 min, 10-100%)