反应详情

A 0.1 mg/ml solution of DTPA anhydride in dry chloroform was prepared and an aliquot containing the desired quantity of DTPA was added to a reaction vessel. The chloroform was removed by evaporation with nitrogen gas at room temperature. A pH 7.0 solution of antibody (approximately 0.5 mg) in 0.05M bicarbonate buffer was added to said reaction vessel to produce a 7:1 molar ratio of anhydride to protein. The solution was incubated with shaking for one minute and the coupled antibody was recovered by passage over a Sephadex G-50 column.

1. additionwas added to a reaction vessel
2. customThe chloroform was removed by evaporation with nitrogen gas at room temperature
3. additionA pH 7.0 solution of antibody (approximately 0.5 mg) in 0.05M bicarbonate buffer was added
4. customreaction vessel
5. customto produce a 7
6. customthe coupled antibody was recovered by passage over a Sephadex G-50 column