反应详情

The obtained wet crystals of Leu-Leu-OBzl (2-MeO-4-OC12OC22) were dissolved in chloroform (13 mL), Fmoc-D-Lys(Boc)-OH (481 mg, 1.03 mmol) and HOBt (13.9 mg, 103 μmol) were added at room temperature, and EDC HCl (216 mg, 1.13 mmol) was further added under ice-cooling. The mixture was warmed to room temperature, stirred overnight and concentrated under reduced pressure. The residue was precipitated with methanol (10 mL) to give Fmoc-D-Lys(Boc)-Leu-Leu-OBzl (2-MeO-4-OC12OC22) (1.14 g, 86% yield relative to Bzl (2-MeO-4-OC12OC22)OH).

1. temperaturecooling
2. concentrationconcentrated under reduced pressure
3. customThe residue was precipitated with methanol (10 mL)