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N-Succinimidyl 3-(2-pyridyldithio)propionate → Spdp dtt
関与物質
| 役割 | 名称 | HCID | 仕込み量 |
|---|---|---|---|
| 反応物 | N-Succinimidyl 3-(2-pyridyldithio)propionate | HCID 100682 | — |
| 反応物 | Succinimidyl 4-(N-maleimidomethyl)cyclohexanecarboxylate | HCID 125175 | — |
| 反応物 | D-1,4-Dithiothreitol | HCID 446094 | — |
| 生成物 | Spdp dtt | HCID 131730073 | — |
反応手順
SPDP-modified/DTT-reduced streptavidin is mixed with SMCC-modified BLA (equimolar ratio) and allowed to react for 1-2 hrs RT (or overnight at 4 degrees). The streptavidin-BLA conjugate product is purified by gel filtration over P-60 resin.
情報源と証拠
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| 特許番号 | US08865891B2 |
| 出典データセット | uspto-grants-2014_10 |
| ORD ID | ord-4477b41de58a4a6a9962a17f986ebdad |
| 出典メモ | uspto-grants-2014_10 |