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HRID538911

反应详情

EQUATION

反应方程式

HRID 538911 的结构方程式

REACTANTS

反应物

3
结构：O=C(CCSSc1ccccn1)ON1C(=O)CCC1=O
HCID100682

N-Succinimidyl 3-(2-pyridyldithio)propionate

C12H12N2O4S2

结构：O=C(ON1C(=O)CCC1=O)C1CCC(CN2C(=O)C=CC2=O)CC1
HCID125175

N-(4-Carboxycyclohexylmethyl)maleimide N-hydroxysuccinimide ester

C16H18N2O6

结构：O[C@H](CS)[C@H](O)CS
HCID446094

Dithiothreitol

C4H10O2S2

PRODUCTS

生成物

1
结构：O=C(CCSSc1ccccn1)ON1C(=O)CCC1=O.O[C@H](CS)[C@H](O)CS
HCID131730073

未命名化合物

PROCEDURE

实验过程

SPDP-modified/DTT-reduced streptavidin is mixed with SMCC-modified BLA (equimolar ratio) and allowed to react for 1-2 hrs RT (or overnight at 4 degrees). The streptavidin-BLA conjugate product is purified by gel filtration over P-60 resin.

WORKUP

后处理

  1. customto react for 1-2 hrs RT (or overnight at 4 degrees)
  2. filtrationThe streptavidin-BLA conjugate product is purified by gel filtration over P-60 resin