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HRID541027

反应详情

EQUATION

反应方程式

HRID 541027 的结构方程式

REACTANTS

反应物

4
结构：NC(=O)CC[C@H](N)C(=O)O
HCID5961

L-Glutamine

C5H10N2O3

结构：CC1(C)S[C@@H]2[C@H](NC(=O)Cc3ccccc3)C(=O)N2[C@H]1C(=O)[O-].[K+]
HCID23664709

Penicillin G potassium

C16H17KN2O4S

结构：Cc1cccc(NC(c2ccc([N+](=O)[O-])cc2)c2ccc3cccc(O)c3n2)n1
HCID131731989

未命名化合物

结构：CN[C@@H]1[C@H](O[C@H]2[C@H](O[C@H]3[C@H](O)[C@@H](O)[C@H](NC(=N)N)[C@@H](O)[C@@H]3NC(=N)N)O[C@@H](C)[C@]2(O)C=O)O[C@@H](CO)[C@H](O)[C@H]1O
HCID178484872

未命名化合物

PRODUCTS

生成物

1
结构：Cc1cccc(NC(c2ccc([N+](=O)[O-])cc2)c2ccc3cccnc3c2O)n1
HCID54589401

未命名化合物

AUXILIARIES

试剂、催化剂与溶剂

1
结构：CS(C)=O
溶剂HCID679

Dimethyl sulfoxide

C2H6OS

2 次

PROCEDURE

实验过程

H9c2 rat embryonic myocardial cells (from ATCC, Rockville, Md., USA) were grown in Dulbecco's modified eagle's medium containing 10% cattle serum, 4 mM L-glutamine (Sigma-Aldrich, Hungary), 100 IU/ml penicillin and 100 ug/ml streptomycin. To the H9c2 supernatant sample, 30 uM of the compound according to Example 1 (7-((6-methylpyridin-2-ylamino (4-nitrophenyl)methyl)quinolin-8-ol from a stock solution containing 30 mM of DMSO (1000-fold dilution) was added before electrophoresis. To the control, DMSO not containing the compound according to Example 1 was added. After electrophoresis, the gel was cut in two halves following renaturation one half was incubated in such a way that 30 uM of the compound, according to Example 1 was added to it at the final concentration, while the second half (control) was incubated without it. The compound according to Example 1 completely inhibited the 72 kDa gelatinase (MMP-2) if the substance was present during incubation. It inhibited the 92 kDa gelatinase (MMP-9) as well, hut to a less extent than the 72 kDa gelatinase,

WORKUP

后处理

  1. additionwas added before electrophoresis
  2. additionwas added
  3. additionwas added to it at the final
  4. concentrationconcentration