L-Glutamine → 7-((6-Methylpyridin-2-ylamino) (4-nitrophenyl)methyl)quinolin-8-ol

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Reaction Steps

H9c2 rat embryonic myocardial cells (from ATCC, Rockville, Md., USA) were grown in Dulbecco's modified eagle's medium containing 10% cattle serum, 4 mM L-glutamine (Sigma-Aldrich, Hungary), 100 IU/ml penicillin and 100 ug/ml streptomycin. To the H9c2 supernatant sample, 30 uM of the compound according to Example 1 (7-((6-methylpyridin-2-ylamino (4-nitrophenyl)methyl)quinolin-8-ol from a stock solution containing 30 mM of DMSO (1000-fold dilution) was added before electrophoresis. To the control, DMSO not containing the compound according to Example 1 was added. After electrophoresis, the gel was cut in two halves following renaturation one half was incubated in such a way that 30 uM of the compound, according to Example 1 was added to it at the final concentration, while the second half (control) was incubated without it. The compound according to Example 1 completely inhibited the 72 kDa gelatinase (MMP-2) if the substance was present during incubation. It inhibited the 92 kDa gelatinase (MMP-9) as well, hut to a less extent than the 72 kDa gelatinase,

Provenance & Evidence