Glycerol → M-Cresol

HRID 574050Notes

Reaction equation

HRID 574050 reaction equation
[C:1]1([OH:7])[CH:6]=[CH:5][CH:4]=[CH:3][CH:2]=1.[ClH:8].[OH-].[Na+:10].O[CH2:12][CH:13]([CH2:15]O)[OH:14]>C([O-])(=O)C.[Zn+2].C([O-])(=O)C.[Zn]>[C:1]1([OH:7])[CH:6]=[CH:5][CH:4]=[CH:3][CH:2]=1.[CH:15]1[C:13]([OH:14])=[CH:12][CH:6]=[CH:1][C:2]=1[CH3:3].[Na+:10].[Cl-:8]

Participants

8

Reactant 3

HCID 753GlycerolHCID 996PhenolHCID 22237896HCID 22237896, compound

Product 3

HCID 342M-CresolHCID 996PhenolHCID 5234Sodium Chloride

Reagents, catalysts & solvents 2

HCID 11192Zinc AcetateCatalystHCID 23994ZincCatalyst

Procedure

An aqueous solution (Stock solution I) of phenol, m-cresol, glycerol and NaCl was prepared together with an aqueous solution (Stock solution II) of LysB29Nε-hexadecandioyl-γ-Glu desB30 human insulin. A fraction of Stock solution I was mixed a fraction of Stock solution II and pH was adjusted to about 7.5. Variable amounts of an aqueous 10 mM zinc acetate solution was added in fractions corresponding to the number of zinc ions per insulin molecule, pH was adjusted to 7.4 using diluted HCl/NaOH and the solution was filtered through a 0.22 μm sterile filter.

Workup

  1. filtrationthe solution was filtered through a 0.22 μm sterile filter

Sources & evidence

ORD record
ord-0e486ba3b6dc4d01895e2207543355f1
Source dataset
uspto-grants-2015_05
Patent number
US09034818B2

Reaction notes

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