HuagongsheHuagongshe Chemistry Print Report · For research reference only
Glycerol → M-Cresol
Participants
| Role | Name | HCID | Charge |
|---|---|---|---|
| Reactant | Glycerol | HCID 753 | — |
| Reactant | Phenol | HCID 996 | — |
| Reactant | HCID 22237896 | HCID 22237896 | — |
| Catalyst | Zinc Acetate | HCID 11192 | — |
| Catalyst | Zinc | HCID 23994 | — |
| Product | M-Cresol | HCID 342 | — |
| Product | Phenol | HCID 996 | — |
| Product | Sodium Chloride | HCID 5234 | — |
Reaction Steps
An aqueous solution (Stock solution I) of phenol, m-cresol, glycerol and NaCl was prepared together with an aqueous solution (Stock solution II) of LysB29Nε-hexadecandioyl-γ-Glu desB30 human insulin. A fraction of Stock solution I was mixed a fraction of Stock solution II and pH was adjusted to about 7.5. Variable amounts of an aqueous 10 mM zinc acetate solution was added in fractions corresponding to the number of zinc ions per insulin molecule, pH was adjusted to 7.4 using diluted HCl/NaOH and the solution was filtered through a 0.22 μm sterile filter.
Provenance & Evidence
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| Patent number | US09034818B2 |
| Source dataset | uspto-grants-2015_05 |
| ORD ID | ord-0e486ba3b6dc4d01895e2207543355f1 |
| Source note | uspto-grants-2015_05 |