Diisopropylcarbodiimide → 4-{Hydroxy-[3-(3-{6-[5-(2-oxo-hexahydro-thieno[3,4-d]imidazol-4-yl)-pentanoylamino]-hexanoylamino}-propoxy)-phenyl]-phenyl-methyl}-benzoic acid succinimidyl ester

Participants

Reaction Steps

To a solution of biotinylated trityl acid (5, 175 mg, 0.244 mM) in anhydrous DMF (3 mL) was added 1,3-diisopropyl carbodiimide (4 mg, 0.35 mM) and stirred the reaction mixture for 5 min. To this reaction mixture was added N-hydroxy succinimide (40 mg, 0.32 mM) and stirred for over night at room temperature. The solvent was removed under high vacuum and the residue obtained was purified by silca gel column chromatography using CH3OH/CH2Cl2, 3:7) mixture as a solvent system. Evaporation of the solvent gave 6 as a white crystalline solid. Yield: 80 mg (41%). 1H-NMR (CD3OD) δ ppm:

Provenance & Evidence