反应详情

To a solution of biotinylated trityl acid (5, 175 mg, 0.244 mM) in anhydrous DMF (3 mL) was added 1,3-diisopropyl carbodiimide (4 mg, 0.35 mM) and stirred the reaction mixture for 5 min. To this reaction mixture was added N-hydroxy succinimide (40 mg, 0.32 mM) and stirred for over night at room temperature. The solvent was removed under high vacuum and the residue obtained was purified by silca gel column chromatography using CH3OH/CH2Cl2, 3:7) mixture as a solvent system. Evaporation of the solvent gave 6 as a white crystalline solid. Yield: 80 mg (41%). 1H-NMR (CD3OD) δ ppm:

1. stirringstirred for over night at room temperature
2. customThe solvent was removed under high vacuum
3. customthe residue obtained
4. customwas purified by silca gel column chromatography
5. customEvaporation of the solvent