反应详情

For the conversion, a mixture of 20 ml of a buffer (100 mM potassium phosphate, pH=8.5, 1 mM MgCl2, 10% glycerol), 20 g 2-chloro-1-(3-chlorophenyl)ethane-1-one dissolved in 80 ml 4-methyl-2-pentanol, 10 mg NAD and 20 000 U recombinant oxidoreductase from Pichia stipidis DSMZ 3651 was incubated at room temperature for 24 h with constant thorough mixing. After 24 h, more than 99% of the 2-chloro-1-(3-chlorophenyl)ethane-1-one used had been reduced. Subsequently, the 4-methyl-2-pentanol phase containing the product was separated from the aqueous phase, filtered, and the product (R)-2-chloro-1-(3-chlorophenyl)ethane-1-ol was obtained by distillation.

1. waitAfter 24 h
2. customwas separated from the aqueous phase
3. filtrationfiltered