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A mixture of (3,5-bis-trifluoromethyl-benzyl)-(6-chloro-1,3-dimethyl-1H-pyrazolo[3,4-b]pyridin-5-ylmethyl)-(2H-tetrazol-5-yl)-amine (8.5 g, 10 mmol) and sodium hydride (0.81 g, 30 mmol) in DMF (150 mL) was stirred at 0° C. for 20 minutes. Thereafter, methyl iodide (3.5 g, 20 mmol) was added and the resulting mixture was stirred at 0-10° C. for 30 minutes. Water (100 mL) was added to the reaction mixture, which was then extracted with ethyl acetate (3×100 mL). The combined organic layers were dried over sodium sulfate and concentrated. The residue was purified by column chromatography over 230-400 mesh silica gel. Elution of the column with 10% acetone in hexanes gave the pure product as a colorless solid (6.3 g), yield: 75%.

1. stirringthe resulting mixture was stirred at 0-10° C. for 30 minutes
2. extractionwas then extracted with ethyl acetate (3×100 mL)
3. dry with materialThe combined organic layers were dried over sodium sulfate
4. concentrationconcentrated
5. customThe residue was purified by column chromatography over 230-400 mesh silica gel
6. washElution of the column with 10% acetone in hexanes