反应详情

温度

60 °C

To a solution of cystine (1 gm, 4.2 mmol) in ammonium hydroxide (10 mL) in a screw-capped vial was added O-methylisourea hydrogen sulfate (1.8 gm, 10 mmol). The vial was sealed and heated to 60° C. for 16 h. The solution was then cooled and the ammonium hydroxide was removed by rotary evaporation. The solid was then dissolved in water (20 mL), filtered through a cotton plug. The product was then isolated by ion exchange chromatography using Bio-Rex 70 resin and eluting with hydrochloric acid (100 mM).

1. customThe vial was sealed
2. temperatureThe solution was then cooled
3. customthe ammonium hydroxide was removed by rotary evaporation
4. dissolutionThe solid was then dissolved in water (20 mL)
5. filtrationfiltered through a cotton plug
6. customThe product was then isolated by ion exchange chromatography
7. washeluting with hydrochloric acid (100 mM)