反应详情

To a solution of guanidino-L-cystine (64 mg, 0.2 mmol) in water (10 mL) was slowly added N,N′-dicyclohexylcarbodiimide (82 mg, 0.4 mmol) and N-hyroxysuccinimide (46 mg, 0.4 mmol) in dioxane (5 mL). After 16 hr, the solution was filtered through a cotton plug and 1,4-bis(3-aminopropyl)piperazine (40 μL, 0.2 mmol) was added. The reaction was allowed to stir at room temperature for 16 h and then the aqueous solution was dialyzed in a 15,000 MW cutoff tubing against water (2×2 l) for 24 h. The solution was then removed from dialysis tubing, filtered through 5 μM nylon syringe filter and then dried by lyophilization to yield 5 mg of polymer.

1. additionwas added
2. customthe aqueous solution was dialyzed in a 15,000 MW cutoff
3. waittubing against water (2×2 l) for 24 h
4. customThe solution was then removed from dialysis tubing
5. filtrationfiltered through 5 μM nylon syringe
6. filtrationfilter
7. customdried by lyophilization