Dicyclohexylcarbodiimide → HCID 178455885, compound

HRID 589429Notes

Reaction equation

HRID 589429 reaction equation
[CH3:1][CH2:2][C@@H:3]([C@@H:5]1[NH:34][C:32](=[O:33])[CH2:31][NH:30][C:28](=[O:29])[C@H:27]2[NH:35][C:36]([C@H:38]([C@H:58]([C@@H:60]([OH:63])[CH2:61][OH:62])[CH3:59])[NH:39][C:40]([C@H:42]3[N:46]([C:47]([C@H:49]([CH2:53][C:54]([NH2:56])=[O:55])[NH:50][C:51](=[O:52])[C@@H:13]([CH2:14][S+:15]([O-:64])[C:16]4[NH:24][C:23]5[CH:22]=[C:21]([OH:25])[CH:20]=[CH:19][C:18]=5[C:17]=4[CH2:26]2)[NH:12][C:10](=[O:11])[CH2:9][NH:8][C:6]1=[O:7])=[O:48])[CH2:45][C@H:44]([OH:57])[CH2:43]3)=[O:41])=[O:37])[CH3:4].[C:65]([O-:73])(=[O:72])[CH2:66][CH2:67][CH2:68][C:69]([O-:71])=[O:70].[OH:74][N:75]1[C:79](=[O:80])[CH2:78][CH2:77][C:76]1=[O:81].C1([N:88]=C=NC2CCCCC2)CCCCC1>CN(C)C=O>[CH3:1][CH2:2][C@@H:3]([C@@H:5]1[NH:34][C:32](=[O:33])[CH2:31][NH:30][C:28](=[O:29])[C@H:27]2[NH:35][C:36]([C@H:38]([C@H:58]([C@@H:60]([OH:63])[CH2:61][OH:62])[CH3:59])[NH:39][C:40]([C@H:42]3[N:46]([C:47]([C@H:49]([CH2:53][C:54]([NH2:56])=[O:55])[NH:50][C:51](=[O:52])[C@@H:13]([CH2:14][S+:15]([O-:64])[C:16]4[NH:24][C:23]5[CH:22]=[C:21]([OH:25])[CH:20]=[CH:19][C:18]=5[C:17]=4[CH2:26]2)[NH:12][C:10](=[O:11])[CH2:9][NH:8][C:6]1=[O:7])=[O:48])[CH2:45][C@H:44]([OH:57])[CH2:43]3)=[O:41])=[O:37])[CH3:4].[OH:74][N:75]=[C:76]([OH:81])[CH2:77][CH2:78][C:79](=[NH:88])[OH:80].[C:65]([OH:73])(=[O:72])[CH2:66][CH2:67][CH2:68][C:69]([OH:71])=[O:70]

Participants

5

Reactant 3

HCID 10868DicyclohexylcarbodiimideHCID 80170N-HydroxysuccinimideHCID 178455886HCID 178455886, compound

Product 1

HCID 178455885HCID 178455885, compound

Reagents, catalysts & solvents 1

HCID 6228DimethylformamideSolvent3×

Procedure

3.4 mg of α-amanitin glutarate (3.3 μmol) was dissolved in 0.05 ml of dry dimethylformamide (DMF), and 2.4 mg (7 eq.) of N-hydroxy-succinimide dissolved in 0.01 ml of DMF were added. After the addition of 1.2 mg of dicyclohexylcarbodiimide in 0.01 ml of DMF the reaction was allowed to proceed for 16 h at RT. The solution was separated from the crystals formed, and the peptide precipitated by the addition of 4 ml of dry diethylether. After centrifugation, the pellet was washed with another 4 ml of ether and centrifuged. The solid was dissolved in 0.1 ml of dimethylformamide and immediately used for the reaction with the antibody solution.

Workup

  1. additionwere added
  2. customThe solution was separated from the crystals
  3. customformed
  4. customthe peptide precipitated by the addition of 4 ml of dry diethylether
  5. washAfter centrifugation, the pellet was washed with another 4 ml of ether
  6. dissolutionThe solid was dissolved in 0.1 ml of dimethylformamide
  7. customimmediately used for the reaction with the antibody solution

Sources & evidence

ORD record
ord-03971c0fdbda4bcdb0815b9e8481398a
Source dataset
uspto-grants-2016_01
Patent number
US09233173B2

Reaction notes

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