反应详情

1,4-Diaminocyclohexane (37.6 mg, 0.33 mmol) was dissolved in dry DMF (3 mL) under nitrogen. Solution of NHS-Biotin (102.3 mg, 0.3 mmol) in dry DMF (3 mL) was added to stirred solution of diamine over a 15 min period. Reaction mixture was stirred for 16 h at ambient temperature. Dilution of reaction mixture with dry Et2O (50 mL) produced white precipitate. Precipitated 19b was filtered, washed with EtOAc (3×20 mL) for removing of NHS and dried in high vacuum.

1. customReaction mixture
2. customproduced white precipitate
3. customPrecipitated 19b
4. filtrationwas filtered
5. washwashed with EtOAc (3×20 mL)
6. customfor removing of NHS
7. customdried in high vacuum