反应详情

A solution of hydrogen chloride in dioxane (0.25 ml, 4M) is added to 24 mg (0.041 mmol) di-tert-butyl (4S)—N-(tert-butoxycarbonyl)-4-[3-tert-butoxy-4-(2-fluoroethoxy)benzyl]-L-glutamate. The reaction mixture is stirred for 4 hours and stored at −25° C. over night. The reaction mixture is concentrated in vacuo. Co. 3 ml dichloro methane is added and the solution is concentrate in vacuo. The last step is repeated. The crude product is purified by HPLC (column, XBrigde, C18, 5 μm 100×30 mm, H2O+0.1% trifluoroacetic acid, acetonitrile, flow 38 mL/min).

1. waitstored at −25° C. over night
2. concentrationThe reaction mixture is concentrated in vacuo
3. addition3 ml dichloro methane is added
4. concentrationthe solution is concentrate in vacuo
5. customThe crude product is purified by HPLC (column, XBrigde, C18, 5 μm 100×30 mm, H2O+0.1% trifluoroacetic acid, acetonitrile, flow 38 mL/min)