3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide → HCID 21584101, compound

HRID 593242Notes

Reaction equation

HRID 593242 reaction equation
[CH3:1][C:2]1[N:6]=[C:5]([N+:7]2[N:11]([C:12]3[CH:17]=[CH:16][CH:15]=[CH:14][CH:13]=3)[N:10]=[C:9]([C:18]3[CH:23]=[CH:22][CH:21]=[CH:20][CH:19]=3)[N:8]=2)[S:4][C:3]=1[CH3:24].[Br-:25]>C(O)(C)C>[Br-:25].[CH3:1][C:2]1[N:6]=[C:5]([N:7]2[N:8]=[C:9]([C:18]3[CH:23]=[CH:22][CH:21]=[CH:20][CH:19]=3)[NH2+:10][N:11]2[C:12]2[CH:17]=[CH:16][CH:15]=[CH:14][CH:13]=2)[S:4][C:3]=1[CH3:24]

Participants

3

Reactant 1

HCID 649653-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide

Product 1

HCID 21584101HCID 21584101, compound

Reagents, catalysts & solvents 1

HCID 3776IsopropanolSolvent

Procedure

Ten μl of thiazolyl blue tetrazolium bromide solution (5 mg/ml in PH 7.4 PBS) was added to each well containing 100 μl of medium and cells, followed by incubation at 37° C. for 4 hours in the dark. Isopropanol (100 μl, 0.04 N HCl) was added to dissolve blue dye. After dissolved completely, absorbance was measured with plate-reader (Synergy 2, BioTek Instrument) at test wavelength 570 nm and reference wavelength 670 nm. Cell viability was determined by absorbance at 570 nm and reported as ratio to control cells (without OGD).

Workup

  1. additioncontaining 100 μl of medium and cells
  2. dissolutionto dissolve blue dye
  3. dissolutionAfter dissolved completely

Sources & evidence

ORD record
ord-7f759d098b764397b56c95e9ade42daa
Source dataset
uspto-grants-2016_01
Patent number
US09242009B2

Reaction notes

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