Phosphate → 7-Acetoacetamido-3(5-methyl-1,3,4-thiadiazol-2-yl)thiomethyl-3-cephem-4-carboxylic acid
Reaction equation
[C:1]([NH:7][CH:8]1[C:23](=[O:24])[N:10]2[C:11]([C:20]([OH:22])=[O:21])=[C:12]([CH2:15]OC(=O)C)[CH2:13][S:14][C@H:9]12)(=[O:6])[CH2:2][C:3]([CH3:5])=[O:4].C(=O)([O-])O.[Na+].[CH3:30][C:31]1[S:35][C:34]([SH:36])=[N:33][N:32]=1.P([O-])([O-])([O-])=O>>[C:1]([NH:7][CH:8]1[C:23](=[O:24])[N:10]2[C:11]([C:20]([OH:22])=[O:21])=[C:12]([CH2:15][S:36][C:34]3[S:35][C:31]([CH3:30])=[N:32][N:33]=3)[CH2:13][S:14][C@H:9]12)(=[O:6])[CH2:2][C:3]([CH3:5])=[O:4]Participants
Procedure
A mixture of 0.356 g. of 7-acetoacetamido-3-acetoxymethyl-3-cephem-4-carboxylic acid, 0.84 g. of sodium hydrogen carbonate, 0.246 g. of 5-methyl-1,3,4-thiadiazol-2-thiol and 20 ml. of a phosphate buffer of pH 6.4 is heated at 60° C for 7.5 hours. After cooling, it is washed with ethyl acetate, adjusted to pH 2 with 50 % phosphoric acid, saturated with sodium chloride and extracted with ethyl acetate. The ethyl acetate layer is dried over magnesium sulfate and concentrated under reduced pressure, followed by cooling with ice. The resultant crystals are recovered by suction-filtration, washed with hexane and dried. The described procedure gives 7-acetoacetamido-3-(5-methyl-1,3,4-thiadiazol-2-yl)thiomethyl-3-cephem-4-carboxylic acid as crystals melting at 177°-180° C (decomp.). Yield 0.274 g. (64 %).
Workup
- additionA mixture of 0.356 g
- temperatureAfter cooling
- washit is washed with ethyl acetate
- extractionextracted with ethyl acetate
- dry with materialThe ethyl acetate layer is dried over magnesium sulfate
- concentrationconcentrated under reduced pressure
- temperatureby cooling with ice
- filtrationThe resultant crystals are recovered by suction-filtration
- washwashed with hexane
- customdried
Sources & evidence
- ORD record
- ord-4502d26896f54d8e880428a73e1204c2
- Source dataset
- uspto-grants-1977_03
- Patent number
- US04012379
Reaction notes
Loading…