HRID605465

反应详情

EQUATION

反应方程式

REACTANTS

反应物

结构：NC(=O)c1ccc[n+]([C@@H]2O[C@H](COP(=O)(O)OP(=O)(O)OC[C@H]3O[C@@H](n4cnc5c(N)ncnc54)[C@H](OP(=O)(O)O)[C@@H]3O)[C@@H](O)[C@H]2O)c1

HCID5886

Adenosine 5′-(trihydrogen diphosphate), 2′-(dihydrogen phosphate), P′→5′-ester with 3-(aminocarbonyl)-1-β-D-ribofuranosylpyridinium

C21H29N7O17P3+

HCID14798

Sodium Hydroxide

HNaO

结构：O=C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO

HCID107526

未命名化合物

结构：CC(C)(C)OC(=O)N[C@@H](Cc1ccccc1)C(=O)CCl

HCID9926241

tert-Butyl [(2S)-4-chloro-3-oxo-1-phenylbutan-2-yl]carbamate

C15H20ClNO3

2 次

PRODUCTS

生成物

结构：CC(C)(C)OC(=O)N[C@@H](Cc1ccccc1)[C@@H](O)CCl

HCID9944139

tert-Butyl [(2S,3R)-4-chloro-3-hydroxy-1-phenylbutan-2-yl]carbamate

C15H22ClNO3

收率 99.6%

AUXILIARIES

试剂、催化剂与溶剂

溶剂HCID1140

Toluene

C7H8

CONDITIONS

反应条件

温度: 30 °C

PROCEDURE

实验过程

Glucose 6 g, NADP 1.4 mg, toluene 0.25 ml, and (3S)-1-chloro-3-tert-butoxycarbonylamino-4-phenyl-2-butanone 2.5 g were added to 50 ml of a cultured medium of E. coli HB101 (pNTOM5G1) prepared in the same manner as in Example 7, and the mixture was stirred at 30° C. while adjusting to pH 6.5 by dropwise addition of 5 M sodium hydroxide. One hour after the start of the reaction, 2.5 g of (3S)-1-chloro-3-tert-butoxycarbonylamino-4-phenyl-2-butanone was added, and the mixture was reacted for 20 hr. After completion of the reaction, the reaction mixture was extracted with toluene, desolvated and the extract was analyzed in the same manner as in Example 8. As a result, (2R,3S)-1-chloro-3-tert-butoxycarbonylamino-4-phenyl-2-butanol (yield 99.6%, diastereomeric excess 99.8% d.e.) was obtained.

WORKUP

后处理

custom(pNTOM5G1) prepared in the same manner as in Example 7
additionwas added
customthe mixture was reacted for 20 hr
customAfter completion of the reaction
extractionthe reaction mixture was extracted with toluene