反应详情

To a solution of 4-[1-phenyl-1-(5-methyl-1,3,4-oxadiazol-2-yl)methylene]-piperidine-1-carboxylic acid tert-butyl ester (0.0348 g, 0.098 mmol) in CH2Cl2 (1 mL) was added TFA (0.5 mL) and the reaction mixture was stirred for 30 min. The solvent was then removed in vacuo and the residual material was dissolved in CHCl3 (1 mL). To this solution was then added 4-methoxy-7-(5-methyl-1,3,4-triazol-2-yl)-6-azaindol-3-yl-oxoacetic acid hydrochloride salt (0.0621 g, 0.206 mmol) and iPrNEt2 (0.18 mL, 1.03 mmol), followed by BOP-Cl (0.0670 g, 0.263 mmol). The mixture was stirred at rt for 2 h and then the solvent was removed in vacuo. The residue was diluted with H2O and extracted with EtOAc (×3). The combined organic layers were washed (H2O, brine), dried (Na2SO4) and concentrated, and the residue was purified by preparative HPLC (NH4OAc) to give the title compound (0.0093 g, 18%) as a colorless solid:

1. customThe solvent was then removed in vacuo
2. dissolutionthe residual material was dissolved in CHCl3 (1 mL)
3. stirringThe mixture was stirred at rt for 2 h
4. customthe solvent was removed in vacuo
5. additionThe residue was diluted with H2O
6. extractionextracted with EtOAc (×3)
7. washThe combined organic layers were washed (H2O, brine)
8. dry with materialdried (Na2SO4)
9. concentrationconcentrated
10. customthe residue was purified by preparative HPLC (NH4OAc)