sodium (4R)-4-[(3R,5S,7R,8R,9R,10S,12S,13R,14R,17R)-3,7,12-trihydroxy-8,10,13-trimethyl-1,2,3,4,5,6,7,9,11,12,14,15,16,17-tetradecahydrocyclopenta[a]phenanthren-17-yl]pentanoate → Palmitic Acid

Participants

Reaction Steps

To 0.5 mL of a cultured cell fraction of Pyrococcus horikoshii JCM 9974 were added 2 mg of each of these substrates, 40 μl of 100 mM sodium cholate and 100 μL of 20 mM calcium chloride. The resultant reaction solution was reacted at 75° C. overnight. From the reaction solution, a fraction containing the fatty acid produced was extracted with isooctane and then subjected to gas chromatography. As a result, a decomposition product including 47-48% of oleic acid, oleic acid, palmitic acid or linolenic acid resulted from the decomposition by phospholipase A2 and 47-48% of palmitic acid, stearic acid, oleic acid or stearic acid resulted from the decomposition by phospholipase A1 was obtained from each of the substrates.

Provenance & Evidence