HRID611460

反应详情

EQUATION

反应方程式

REACTANTS

反应物

结构：C[C@H](CCC(=O)[O-])[C@H]1CC[C@@H]2[C@]1(C)[C@@H](O)C[C@@H]1[C@@]3(C)CC[C@@H](O)C[C@H]3C[C@@H](O)[C@@]21C.[Na+]

HCID44582063

未命名化合物

PRODUCTS

生成物

HCID985

Hexadecanoic acid

C16H32O2

收率 47%

HCID5281

Octadecanoic acid

C18H36O2

$结构：CCCCCCCC/C=C\CCCCCCCC(=O)O$

HCID445639

Oleic acid

C18H34O2

AUXILIARIES

试剂、催化剂与溶剂

溶剂HCID5284359

未命名化合物

PROCEDURE

实验过程

To 0.5 mL of a cultured cell fraction of Pyrococcus horikoshii JCM 9974 were added 2 mg of each of these substrates, 40 μl of 100 mM sodium cholate and 100 μL of 20 mM calcium chloride. The resultant reaction solution was reacted at 75° C. overnight. From the reaction solution, a fraction containing the fatty acid produced was extracted with isooctane and then subjected to gas chromatography. As a result, a decomposition product including 47-48% of oleic acid, oleic acid, palmitic acid or linolenic acid resulted from the decomposition by phospholipase A2 and 47-48% of palmitic acid, stearic acid, oleic acid or stearic acid resulted from the decomposition by phospholipase A1 was obtained from each of the substrates.

WORKUP

后处理

customThe resultant reaction solution
customwas reacted at 75° C. overnight
customproduced
extractionwas extracted with isooctane

来源与证据

来源类型: Open Reaction Database
ORD 记录: ord-c8c00cdd1b30490b9b3d5e825036be32
来源数据集: uspto-grants-1999_12
DOI: 10.6084/m9.figshare.5104873.v1
专利: US06001626
来源说明: uspto-grants-1999_12

反应 SMILES

C[C@@H:2]([C@@H:8]1[C@@:12]2(C)[C@@H:13](O)[CH2:14][C@@H:15]3[C@@:20]4(C)[CH2:21]C[C@@H](O)C[C@H:19]4[CH2:18][C@@H:17](O)[C@@:16]3(C)[C@@H:11]2[CH2:10][CH2:9]1)[CH2:3][CH2:4][C:5]([O-:7])=[O:6].[Na+]>[Cl-].[Ca+2].[Cl-]>[C:5]([OH:7])(=[O:6])[CH2:4][CH2:3][CH2:2][CH2:8][CH2:9][CH2:10][CH2:11][CH2:12][CH2:13][CH2:14][CH2:15][CH2:16][CH2:17][CH2:18][CH3:19].[C:5]([OH:7])(=[O:6])[CH2:4][CH2:3][CH2:2][CH2:8][CH2:9][CH2:10][CH2:11]/[CH:12]=[CH:13]\[CH2:14][CH2:15][CH2:16][CH2:17][CH2:18][CH2:19][CH2:20][CH3:21].[C:5]([OH:7])(=[O:6])[CH2:4][CH2:3][CH2:2][CH2:8][CH2:9][CH2:10][CH2:11][CH2:12][CH2:13][CH2:14][CH2:15][CH2:16][CH2:17][CH2:18][CH2:19][CH2:20][CH3:21]