反应详情

温度

110 °C

1-Benzyl-5-chloro-2-[1-(tetrahydro-2H-pyran-2-yl)-1H-pyrazol-5-yl]-1H-pyrrolo[3,2-b]pyridine (0.075 g, 0.19 mmol, from Step 3), di-tert-butyl hydrazine-1,2-dicarboxylate (0.049 g, 0.21 mmol, Aldrich) and Cs2CO3 (0.062 g, 0.19 mmol, Aldrich) were combined in toluene (1.7 mL) and dicyclohexyl(2′,4′,6′-triisopropylbiphenyl-2-yl)phosphine-(2′-aminobiphenyl-2-yl)(chloro)palladium (1:1) (0.015 g, 0.019 mmol, Aldrich) was added. The mixture was degassed by a stream of nitrogen through the solution for 10 minutes. The vial was sealed and heated to 110° C. for 3 hours. Additional reagents and catalyst (same of each as the initial quantities) were added, the mixture was degassed again and the reaction mixture was then heated in the microwave to 140° C. for 10 minutes. After cooling to room temperature, the reaction mixture was partitioned between EtOAc and water, the aqueous layer was extracted three times, and the combined extracts were dried over sodium sulfate, filtered and concentrated. The product was purified via preparative HPLC-MS (C18 eluting with a gradient of MeCN and H2O containing 0.15% NH4OH). Yield: (45 mg, 40%).

1. customThe mixture was degassed by a stream of nitrogen through the solution for 10 minutes
2. customThe vial was sealed
3. additionAdditional reagents and catalyst (same of each as the initial quantities) were added
4. customthe mixture was degassed again
5. temperaturethe reaction mixture was then heated in the microwave to 140° C. for 10 minutes
6. temperatureAfter cooling to room temperature
7. customthe reaction mixture was partitioned between EtOAc and water
8. extractionthe aqueous layer was extracted three times
9. dry with materialthe combined extracts were dried over sodium sulfate
10. filtrationfiltered
11. concentrationconcentrated
12. customThe product was purified via preparative HPLC-MS (C18
13. washeluting with a gradient of MeCN and H2O containing 0.15% NH4OH)