(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal → D-xylonic acid

Participants

Reaction Steps

H3494 produced extracellularly 2.7 g xylonate I−1 after 144 h at pH 5.5 (Table 1, FIG. 10). Xylonate began to accumulate in the culture supernatant after the initial glucose supply had been either consumed or converted to ethanol. The xylonate production rate (33 mg xylonate I−1 h−1 or 10 mg xylonate [g biomass]−1 h−1) decreased after the ethanol had been consumed (13 mg xylonate I−1 h−1 or 3 mg xylonate [g biomass]−1 h−1; FIG. 10). The yield of xylonate on xylose was approximately 0.47 g xylonate [g xylose]−1. In addition to xylonate, xylitol, glycerol and acetate were also produced. Glycerol and acetate were subsequently consumed, but the xylitol was not.

Provenance & Evidence