(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal → D-xylonic acid

H3494 produced extracellularly 2.7 g xylonate I−1 after 144 h at pH 5.5 (Table 1, FIG. 10). Xylonate began to accumulate in the culture supernatant after the initial glucose supply had been either consumed or converted to ethanol. The xylonate production rate (33 mg xylonate I−1 h−1 or 10 mg xylonate [g biomass]−1 h−1) decreased after the ethanol had been consumed (13 mg xylonate I−1 h−1 or 3 mg xylonate [g biomass]−1 h−1; FIG. 10). The yield of xylonate on xylose was approximately 0.47 g xylonate [g xylose]−1. In addition to xylonate, xylitol, glycerol and acetate were also produced. Glycerol and acetate were subsequently consumed, but the xylitol was not.

1. customeither consumed
2. customThe xylonate production rate (33 mg xylonate I−1 h−1 or 10 mg xylonate [g biomass]−1 h−1)
3. customhad been consumed (13 mg xylonate I−1 h−1 or 3 mg xylonate [g biomass]−1 h−1; FIG. 10)
4. additionIn addition to xylonate, xylitol, glycerol and acetate
5. customwere also produced
6. customGlycerol and acetate were subsequently consumed

ORD record

ord-db0c185aadcb4de5b0af3722dee80205

Source dataset

uspto-grants-2016_04

DOI

10.6084/m9.figshare.5104873.v1

Patent number

US09315834B2