Acetic Acid → HCID 86704522, compound

Tetra-butylammonium fluoride (1M solution in THF; 37.2 mL, 37.2 mmol) was added to a stirred solution of 4,4-bis(((tert-butyldimethylsilyl)oxy)methyl)-8-nitro-4H-benzo[b]tetrazolo[1,5-d][1,4]oxazine (6.3 g, 12.4 mmol) and AcOH (2.1 mL, 37.2 mmol) in THF (200 mL). The mixture was stirred at room temperature for 2 hr then concentrated under vacuum. The residue was partitioned between EtOAc (200 mL) and H2O (150 mL) and the organic layer was washed with brine (1×100 mL), dried (Na2SO4), filtered and the solvent removed under vacuum to leave a crude residue. The residue was dry-loaded on to silica gel then purified by column chromatography on silica gel using DCM/MeOH (1:0 to 92:8) as eluent to provide the desired compound (1.6 g). The desired compound was dissolved in EtOAc (150 mL) and washed with H2O (12×100 mL). The organic layer was dried (Na2SO4), filtered and the solvent removed under vacuum to leave the product (0.9 g) as a solid.

1. concentrationthen concentrated under vacuum
2. customThe residue was partitioned between EtOAc (200 mL) and H2O (150 mL)
3. washthe organic layer was washed with brine (1×100 mL)
4. dry with materialdried (Na2SO4)
5. filtrationfiltered
6. customthe solvent removed under vacuum
7. customto leave a crude residue
8. customthen purified by column chromatography on silica gel

ORD record

ord-b317149ef5d74e8792aa72cd7cac30cc

Source dataset

uspto-grants-2016_04

DOI

10.6084/m9.figshare.5104873.v1

Patent number

US09321763B2