Participants
| Role | Name | HCID | Charge |
|---|
| Reactant | Sodium Hydroxide | HCID 14798 | — |
| Reactant | N-Acetyl-L-leucine | HCID 70912 | — |
| Reactant | N-Acetylisoleucine | HCID 7036275 | — |
| Solvent | Water | HCID 962 | — |
| Product | L-Leucine | HCID 6106 | — |
Reaction Steps
86.6 grams of this mixture of equal parts of N-acetyl-leucine and N-acetyl-isoleucine were suspended in 400 ml of water and adjusted to pH 7.0 with 50% aqueous sodium hydroxide. There were added 50 mg of CoCl2X6H2O and 50 mg of commerical pig kidney acylase (1200 U/mg) and the mixture was filled up to 500 ml with water. The clear solution was stirred at 36° C. for 6 hours. By filtering with suction, washing and drying there were obtained 17.9 grams of pure L-leucine having a rotary value of [α]D25 =+15.9°.
Provenance & Evidence
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|
| Patent number | US04562153 |
|---|
| Source dataset | uspto-grants-1985_12 |
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| ORD ID | ord-1d3a85980dba4ee1a0ce9052fe130086 |
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| Source note | uspto-grants-1985_12 |
|---|
