反应详情

温度

36 °C

86.6 grams of this mixture of equal parts of N-acetyl-leucine and N-acetyl-isoleucine were suspended in 400 ml of water and adjusted to pH 7.0 with 50% aqueous sodium hydroxide. There were added 50 mg of CoCl2X6H2O and 50 mg of commerical pig kidney acylase (1200 U/mg) and the mixture was filled up to 500 ml with water. The clear solution was stirred at 36° C. for 6 hours. By filtering with suction, washing and drying there were obtained 17.9 grams of pure L-leucine having a rotary value of [α]D25 =+15.9°.

1. additionThere were added 50 mg of CoCl2X6H2O and 50 mg of commerical pig kidney acylase (1200 U/mg)
2. filtrationBy filtering with suction
3. washwashing
4. customdrying there